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KMID : 0894520080120030297
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Development & Reproduction
2008 Volume.12 No. 3 p.297 ~ p.303
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Cloning and Functional Studies of Pro-Apoptotic MCL-1ES BH3M
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Kim Jae-Hong
Park Mi-Ra
Ha Hye-Jeong
Lee Kang-Seok
Bae Jee-Hyeon
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Abstract
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BCL-2 family members are essential protein for the regulation of cell death and survival consisting both antiapoptotic and pro-apoptotic proteins. In the present study, we designed and cloned a new apoptotic molecule MCL-1ES BH3M coding a modified protein of MCL-1L. Compared to MCL-1L protein, MCL-1ES BH3M lacks the PEST motifs known to be involved in MCL-1L protein degradation and has seven mutated residues in BH3 domain critical for dimerization with BCL-2 family members. Overexpression of MCL-1ES BH3M induced death of different cells, and its cell killing effect was not blocked by forced expression of the pro-survival protein MCL-1L. Expression of MCL-1ES BH3M protein led to the activation of caspase 9 and caspase 3, suggesting apoptotic cell death, and confocal fluorescent microscopic analyses showed that MCL-1ES BH3M was partially localized in mitochondria. In conclusion, we reported a new apoptotic molecule and determined its cell death activity in cells.
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KEYWORD
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Apoptosis, MCL-1L, MCL-1ES BH3M, BH3 domain, BCL-2 family
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